Generator

Part:BBa_K2694000

Designed by: Jessica Larocque   Group: iGEM18_NDC-HighRiverAB   (2018-10-10)


EstA generator under control of pLac promoter

The esterase in this circuit is an enzyme from Pseudomonas aeruginosa. The function of the esterase is to cleave ester bonds, such as those found in triglycerides. The production of the protein is under the control of a pLac promoter (BBa_R0011), with a strong ribosome binding site (BBa_B0034). At the end of the circuit there is a double terminator (BBa_B0010 and BBa_B0012). The circuit was designed by the 2018 NDC-HighRiverAB team to produce the esterase protein for testing purposes. Our team introduced this circuit to DH5α and tested its activity towards two different substrates (4-nitrophenyl palmitate and 4-nitrophenyl octanoate).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 296
    Illegal BglII site found at 443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal NgoMIV site found at 682
    Illegal NgoMIV site found at 820
    Illegal NgoMIV site found at 1360
    Illegal NgoMIV site found at 1729
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None